Journal: Tissue Engineering. Part A

Article Title: Investigation on Ciliary Functionality of Different Airway Epithelial Cell Lines in Three-Dimensional Cell Culture

doi: 10.1089/ten.tea.2019.0188

Figure Lengend Snippet: Qualitative Overview of the Mucociliary Phenotype of Three-Dimensional Airway Mucosa Tissue Models Used in This Study

Article Snippet: The human airway epithelial cell lines Cl-huAEC (Inscreenex GmbH, Braunschweig, Germany) and Calu-3 (ATCC ® HTB-55 TM , Manassas, VA) were handled according to provider's instructions and cultured in huAEC (INS-ME-1013-500ml; Inscreenex) and minimum essential medium (No. 11095080; Thermo Fisher Scientific, Waltham, MA) supplemented with 10% fetal calf serum (FCS) and 1% sodium pyruvate, respectively.

Techniques:

Journal: Tissue Engineering. Part A

Article Title: Investigation on Ciliary Functionality of Different Airway Epithelial Cell Lines in Three-Dimensional Cell Culture

doi: 10.1089/ten.tea.2019.0188

Figure Lengend Snippet: H&E staining of 3D airway mucosa tissue models using different respiratory epithelial cell lines. 3D cell culture of HBEC3-KT-based models reveals a polarized epithelial cell layer (A) containing kinocilia-like structures on the apical surface (A , inset ) . VA10-based models show a rather undifferentiated epithelial layer (B) . Calu-3-based models show cell cluster formation (C) . Cl-huAEC-based models show a polarized cell monolayer on the apical surface (D) . Calu-3-, VA10-, and Cl-huAEC-based models do not show kinocilia-like structures (B–D) . Scale bars: 100 μm. 3D, three dimensional; H&E, hematoxylin and eosin.

Article Snippet: For generation of the CI-huAEC cell line, primary human bronchial epithelial cells were obtained from Promocell GmbH.

Techniques: Staining, Cell Culture

Journal: Tissue Engineering. Part A

Article Title: Investigation on Ciliary Functionality of Different Airway Epithelial Cell Lines in Three-Dimensional Cell Culture

doi: 10.1089/ten.tea.2019.0188

Figure Lengend Snippet: Immunofluorescent staining of vimentin, CK5, CK18, MUC5AC, and MUC5B in 3D airway mucosa tissue models. Vimentin is localized in the SIS of each cell line-based model (A, E, I, M) . 3D tissue models based on HBEC3-KT, VA10, and Cl-huAEC show CK5 ( red ) and CK18 ( green ) expression in the epithelial compartment (B, F, N) , whereas Calu-3-based models show only CK18 expression (J) . MUC5AC and MUC5B are identified in HBEC3-KT- (C, D) , VA10- (G, H), and Calu-3-based models (K, L), but are not visible in Cl-huAEC-based models (O, P) . Scale bars: 50 μm. CK, cytokeratin; MUC, mucin; SIS, small intestinal submucosa.

Article Snippet: For generation of the CI-huAEC cell line, primary human bronchial epithelial cells were obtained from Promocell GmbH.

Techniques: Staining, Expressing

Journal: Tissue Engineering. Part A

Article Title: Investigation on Ciliary Functionality of Different Airway Epithelial Cell Lines in Three-Dimensional Cell Culture

doi: 10.1089/ten.tea.2019.0188

Figure Lengend Snippet: Qualitative Overview of the Mucociliary Phenotype of Three-Dimensional Airway Mucosa Tissue Models Used in This Study

Article Snippet: For generation of the CI-huAEC cell line, primary human bronchial epithelial cells were obtained from Promocell GmbH.

Techniques:

Journal: Tissue Engineering. Part A

Article Title: Investigation on Ciliary Functionality of Different Airway Epithelial Cell Lines in Three-Dimensional Cell Culture

doi: 10.1089/ten.tea.2019.0188

Figure Lengend Snippet: Ultrastructure of 3D airway mucosa tissue models using different airway epithelial cell lines. HBEC3-KT-based models show kinocilia on the apical surface ( black arrows ). Basal bodies are clearly visible ( white arrows ) and microvilli ( black arrowheads ) line the epithelial surface (A) . Tissue models generated with VA10 (B) , Calu-3 (C) or Cl-huAEC (D) show microvilli and tight junctions ( arrows in insets B – D , respectively ) on the epithelial cell surface. Scale bars: 3 μm (A–D) , 0.5 μm ( insets ).

Article Snippet: For generation of the CI-huAEC cell line, primary human bronchial epithelial cells were obtained from Promocell GmbH.

Techniques: Generated

Journal: Tissue Engineering. Part A

Article Title: Investigation on Ciliary Functionality of Different Airway Epithelial Cell Lines in Three-Dimensional Cell Culture

doi: 10.1089/ten.tea.2019.0188

Figure Lengend Snippet: Qualitative Overview of the Mucociliary Phenotype of Three-Dimensional Airway Mucosa Tissue Models Used in This Study

Article Snippet: The human airway epithelial cell lines Cl-huAEC (Inscreenex GmbH, Braunschweig, Germany) and Calu-3 (ATCC ® HTB-55 TM , Manassas, VA) were handled according to provider's instructions and cultured in huAEC (INS-ME-1013-500ml; Inscreenex) and minimum essential medium (No. 11095080; Thermo Fisher Scientific, Waltham, MA) supplemented with 10% fetal calf serum (FCS) and 1% sodium pyruvate, respectively.

Techniques: